El objetivo general del Laboratorio de Estudios en Enfermedad de Chagas es contribuir al mejor conocimiento de las interrelaciones neuro-inmuno-endócrinas y metabólicas en esta patología, como también evaluar vacunas experimentales basadas en antígenos del parásito Trypanosoma cruzi.

Investigadores:

 

Dra. Ana Rosa Perez

Dr. Andrés Alloatti

Dra. Silvina Villar

Dra. Florencia González

Dr. Eduardo Roggero

Dr. Oscar Bottasso

Dr. Héctor Dávila

Becarios y Doctorandos:

Lic. en Biotecnología M. F. Pacini (Becaria Doctoral CONICET)

Lic. Camila Bulfoni (Becaria Doctoral ANCYPT))

Lic. Brenda Dinatale (Becaria Doctoral CONICET)

Lic. Lucía Biscari (Becaria Doctoral CONICET)

Lic. Cintia Kaufman (Becaria Doctoral CONICET)

Lic. Cecilia Farré (Doctorando)

Tesinistas:

Antonella Pacini

Paula Cambronera

Irma Quattoni

 

Líneas de Investigación:

  • Análisis de las alteraciones inmuno-neuroendócrinas en individuos chagásicos con diferente grado de compromiso cardíaco y en modelos experimentales. Responsable: Dra. A.R. Pérez E-mail: perez@idicer-conicet.gob.ar
  • Evaluación del rol de la presentación antigénica en el desarrollo de la Enfermedad de Chagas. Responsable: Dr. Andrés Alloatti E-mail: alloatti@idicer-conicet.gob.ar
  • Estudio de las alteraciones tímicas en la infección causada por T. cruzi. Responsable: Dra. A.R. Pérez E-mail: perez@idicer-conicet.gob.ar
  • Estudio del papel de los adipocitos, las adipocinas, PPARγ y otros mediadores metabólicos sobre la respuesta inmune y el compromiso cardíaco. Responsable: Dra. A.R. Pérez E-mail: perez@idicer-conicet.gob.ar
  • Diseño de vacunas de utilidad en la Enfermedad de Chagas y evaluación de antígenos de T. cruzi como potenciales adyuvantes. Responsable: Dra. A.R. Pérez E-mail: perez@idicer-conicet.gob.ar
  • Fisiopatología de las glándulas adrenales durante la infección por T. cruzi. Responsable: Dra. S. Villar. E-mail: villar@idicer-conicet.gob.ar

Publicaciones relacionadas (últimos años):

Chagas disease reactivation in rheumatologic patients: Association with immunosuppressive therapy and humoral response. Ringer A, Ruffino JP, Leiva R Cuadranti N, Argento C, Martinez MF, Rolla I, Chulibert S, Carbone D, Palatnik M, Cortese N, Lagrutta M, Cordoba L, Sciarrata P, Gonzalez FB, Pacini MF, Villar S, Aguila D, Bottasso O, Pérez AR, Abdala M. Clinical Rheumatology 2021 doi: 10.1007/s10067-021-05581-2.

The thymus in Chagas Disease: molecular interactions involved in abnormal T-cell migration and differentiation. Ana Rosa Pérez, Juliana de Meis, Maria Cecilia Rodriguez Galán and Wilson Savino. Frontiers in Immunology Front Immunol. 2020 Sep 2;11:1838. doi: 10.3389/fimmu.2020.01838. eCollection 2020.

Activation of PPARγ reduces antioxidant-induced hypercorticoidism by down-regulating MC2R expression into the adrenal glands. Raíssa D. Ventura, Amanda S. Chaves, Nathalia S. Magalhães, Florencia B. Gonzalez, Maria Florencia Pacini, Ana Rosa Pérez, Patrícia M. R. Silva, Marco A. Martins, and Vinicius F. Carvalho. Free Radical Biology and Medicine (2020 Jun 20;156:137-143).

Immune-neuroendocrine and metabolic disorders in human and experimental cruzi infection: New clues for understanding Chagas disease pathology. González FB, Villar SR, Pacini MF, Bottasso OA, Pérez AR. Biochim Biophys Acta Mol Basis Dis. 2020;1866(3):165642.

Evidence in favor of an alternative glucocorticoid synthesis pathway during acute experimental Chagas disease. Esdras Da Silva Barbosa, Eduardo Angel Roggero, Florencia Belen Gonzalez, Rocio del Valle Fernandez, Vinicius Frias Carvalho, Oscar Bottasso and Ana Rosa Pérez, Silvina Raquel Villar. Front Endocrinol (Lausanne). Enero 2020;10:866. doi: 10.3389/fendo.2019.00866 .eCollection 2019.

Diminished Prolinemia in Chronic Chagasic Patients. A New Clue for Disease Pathology? Sandra Carla Rocha; Ana Rosa Pérez; Juan Beloscar; Oscar Bottasso; Ariel Mariano Silber. Molecules. 2019 Aug 30;24(17). pii: E3167.

Ana Rosa Pérez, Daniella Areas Mendes-da-Cruz, Vincent Geenen and Wilson Savino. Editorial: Hormones, Neurotransmitters, and T-Cell Development in Health and Disease. Endocrinol. 2019 doi.org/10.3389/fendo.2019.00454.

Immune response triggered by Trypanosoma cruzi infection strikes adipose tissue homeostasis altering lipid storage, enzyme profile and adipokine expresión. Florencia B. González, Silvina R. Villar, Judith Toneatto, María F. Pacini, Julia Márquez, Luciano D’Attilio, Oscar A. Bottasso, Graciela Piwien‑Pilipuk, Ana R. Pérez. Med Microbiol and Immunology. Med Microbiol Immunol. 2019 Oct;208(5):651-666.

Immune-endocrine interactions related to a high risk of infections in chronic metabolic diseases: The role of PPAR gamma.

Silva AR, Gonçalves-de-Albuquerque CF, Pérez AR, Carvalho VF.Eur J Pharmacol. 2019 Apr 8;854:272-281. doi: 10.1016/j.ejphar.2019.04.008.

Disruption of tumor necrosis factor alpha receptor 1 signaling accelerates NAFLD progression in mice upon a high-fat diet.

Lambertucci F, Arboatti A, Sedlmeier MG, Motiño O, Alvarez ML, Ceballos MP, Villar SR, Roggero E, Monti JA, Pisani G, Quiroga AD, Martín-Sanz P, Carnovale CE, Francés DE, Ronco MT. J Nutr Biochem. 2018 Aug;58:17-27. doi: 10.1016/j.jnutbio.2018.04.013.

Dysregulated network of immune, endocrine and metabolic markers is associated to more severe human chronic Chagas cardiomyopathy”. González Florencia Belén, Villar Silvina, D’Attilio Luciano, Leiva Rodolfo, Marquez Julia, Lioi Susana, Beloscar Juan, Bottasso Oscar, Pérez Ana Rosa. Neuroimmunomodulation. 2018;25(3):119-128. doi: 10.1159/000491699. Link: https://www.ncbi.nlm.nih.gov/pubmed/30253402

Immune response triggered by Trypanosoma cruzi infection strikes adipose tissue homeostasis altering lipid storage, enzyme profile and adipokine expresión. Florencia B. González, Silvina R. Villar, Judith Toneatto, María F. Pacini, Julia Márquez, Luciano D’Attilio, Oscar A. Bottasso, Graciela Piwien‑Pilipuk, Ana R. Pérez. Medical Microbiology and Immunology. doi: 10.1007/s00430-018-0572-z

The mucosal adjuvant 3´ 5´- cyclic di adenosine monophosphate (c-di-AMP) to design a mucosal vaccine prototype”. Ingrid Quintana, Martín Espariz, Jörg Stülke, Silvina Villar, Florencia B. González, Florencia Pacini, Gabriel Cabrera, Ivan Bontempi, Estefanía Procheto, Ana Rosa Perez, Ivan Marcipar, Victor Blancato and Christian Magni. Frontiers in Microbiology. doi: 10.3389/fmicb.2018.02100.

Trans-sialidase-based vaccine protects against Trypanosoma cruzi infection, not only inducing an effector immune response but also affecting cells with regulatory/suppressor phenotype. E. Prochetto, C. Roldán, I. Bontempi, D. Bertona, L. Peverengo, M. Vicco, L.M. Rodeles, A.R. Pérez, I. Marcipar, G. Cabrera. Oncotarget, 2017; 8(35): 58003–58020.

Death of adrenocortical cells during murine acute T. cruzi infection is not associated with TNF-R1 signaling but with the type II pathway of Fas-mediated apoptosis. AR Pérez, F Lambertucci, FB González, EA Roggero, OA. Bottasso, J de Meis, MT Ronco, SR Villar. Brain Behav Immun. 2017; 65:284-295.

The sympathetic nervous system affects the susceptibility and course of Trypanosoma cruzi infection. Roggero E, Pérez AR, Pollachini N, Villar SR, Wildmann J, Besedovsky H, Del Rey A. Brain Behav Immun. 2016 Jul 30. pii: S0889-1591(16)30357-9. doi: 10.1016/j.bbi.2016.07.163.

Evasion and immuno-endocrine regulation in parasite infection: Two sides of the same coin in Chagas disease? A. Morrot, S. R. Villar, F. B González, A. R. Pérez. Front. Microbiol., 23 May 2016 |

Role of Trypanosoma cruzi Trans-sialidase on the escape from host immune surveillance. Nardy AF, Freire-de-Lima CG, Pérez AR, Morrot A. Front Microbiol. 2016 Mar 23;7:348. doi: 10.3389/fmicb.2016.00348. eCollection 2016. Review.

Trypanosoma cruzi Experimental Infection Impacts on the Thymic regulatory T cell compartment.  González FB, Calmon-Hamaty F, Nô Seara Cordeiro S, Fernández Bussy R, Spinelli SV, D’Attilio L, Bottasso O, Savino W, Cotta-de-Almeida V, Villar SR, Pérez AR. PLoS Negl Trop Dis. 2016 Jan 8;10(1):e0004285.

Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection? Barreto-de-Albuquerque J, Silva-dos-Santos D, Pérez AR, Berbert LR, de Santana-van-Vliet E, Farias-de-Oliveira DA, Moreira OC, Roggero E, de Carvalho-Pinto CE, Jurberg J, Cotta-de-Almeida V, Bottasso O, Savino W, de Meis J. PLoS Negl Trop Dis. 2015 Jun 19;9(6):e0003849.

Efficacy of a Trypanosoma Trans-sialidase-ISCOMATRIX subunit vaccine candidate to protect against experimental Chagas disease. Bontempi IA, Vicco MH, Cabrera G, Villar SR, González FB, Roggero EA, Ameloot P, Callewaert N, Pérez AR, Marcipar IS. Vaccine (2015) 33(10):1274-83.

Immunoendocrine dysbalance during uncontrolled T. cruzi infection is associated with the acquisition of a Th-1-like phenotype by Foxp3+ T cells. González FB, Villar SR, Fernández Bussy R, Martin GH, Pérol L, Manarin R, Spinelli SV, Pilon C, Cohen JL, Bottasso OA, Piaggio E, Pérez AR. Brain Behav Immun. 2015 Mar;45:219-32.

Early double-negative thymocyte export in Trypanosoma cruzi infection is restricted by sphingosine receptors and associated with human Chagas disease. Lepletier A, Almeida L de, Silva Sampaio L de, Paredes B, Santos L, González FB, Beloscar J, Bottasso O, Einicker-Lamas M, Pérez AR, Savino W, Morrot A. PLoS Negl Trop Dis (2014),8(10):e3203.